Journal: bioRxiv

Article Title: Selective germline genome edited pigs and their long immune tolerance in Non Human Primates

doi: 10.1101/2020.01.20.912105

Figure Lengend Snippet: (a ) WT pig fibroblasts were stained with the xenoantigens related antibody of GGTA, B4Gal and Camh, xenoantigens binding WT fibroblasts was noted by the marked increase in fluorescence compared to non-specific Isotype antibody stained cells.(b)SGGEP pig fibroblasts were stained with the xenoantigens related antibody of GGTA, B4Gal and Camh, xenogens binding WT fibroblasts was noted by the no increase in fluorescence compared to Isotype antibody stained cells.(c) WT pig fibroblasts were stained with the human proteins related antibodies: from top TFPI,THBD,HLAE,CD39,CD47,CD59,CD55,CD46, no increase in fluorescence in the cells compared to isotype antibody stained cells indicating no expression of these protein in WT fibroblasts. (d) SGGEP pig fibroblasts were stained with the human proteins related antibodies: from top TFPI,THBD,HLAE,CD39,CD47,CD59,CD55,CD46, increase in fluorescence staining in the cells compared to Isotype antibody stained cells indicating expression of these proteins.

Article Snippet: Anti-Human CD55, CD59, CD46, CD47, CD39, and FITC conjugated antibodies were purchased from BD, USA.

Techniques: Staining, Binding Assay, Fluorescence, Expressing

Journal: Clinical and Experimental Immunology

Article Title: Complement regulatory proteins in kidneys of patients with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis

doi: 10.1111/cei.13051

Figure Lengend Snippet: Immunohistochemistry staining for CD46, CD55 and CD59 in glomeruli of renal specimens. (a–c) Immunohistochemical staining of CD46, CD55 and CD59 in glomeruli of a normal control. (d–f) Immunohistochemical staining of CD46, CD55 and CD59 in glomeruli of patient with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis. (g–i) Comparison of the mean optical density of CD46, CD55 and CD59 in glomeruli of patients with ANCA‐associated vasculitis to that of normal controls. [Colour figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Detection of complement regulatory proteins expression in kidney by immunohistochemistry Immunohistochemical staining was performed for CD46, CD55 and CD59 on 4‐μm deparaffinized sections of formaldehyde‐fixed renal tissue using rabbit anti‐human CD46 polyclonal antibodies (Abcam, Cambridge, UK; ab108307), mouse anti‐human CD55 monoclonal antibodies (Abcam; ab1422) and rabbit anti‐human CD59 polyclonal antibodies (Sigma‐Aldrich, St Louis, MO, USA; HPA026494) as primary antibodies.

Techniques: Immunohistochemistry, Staining, Immunohistochemical staining

Journal: Clinical and Experimental Immunology

Article Title: Complement regulatory proteins in kidneys of patients with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis

doi: 10.1111/cei.13051

Figure Lengend Snippet: Immunohistochemistry staining for CD46, CD55 and CD59 in the tubulointerstitial compartment of renal specimens. (a–c) Immunohistochemical staining of CD46, CD55 and CD59 in the tubulointerstitial compartment of a normal control. (d–f) Immunohistochemical staining of CD46, CD55 and CD59 in the tubulointerstitial compartment of patient with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis. [Colour figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Detection of complement regulatory proteins expression in kidney by immunohistochemistry Immunohistochemical staining was performed for CD46, CD55 and CD59 on 4‐μm deparaffinized sections of formaldehyde‐fixed renal tissue using rabbit anti‐human CD46 polyclonal antibodies (Abcam, Cambridge, UK; ab108307), mouse anti‐human CD55 monoclonal antibodies (Abcam; ab1422) and rabbit anti‐human CD59 polyclonal antibodies (Sigma‐Aldrich, St Louis, MO, USA; HPA026494) as primary antibodies.

Techniques: Immunohistochemistry, Staining, Immunohistochemical staining

Journal: Clinical and Experimental Immunology

Article Title: Complement regulatory proteins in kidneys of patients with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis

doi: 10.1111/cei.13051

Figure Lengend Snippet: Immunohistochemical staining of CD46, CD55 and CD59 on inflammatory cells in renal specimen of a patient with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis. (a) Immunohistochemical staining of CD46 on inflammatory cells in kidney of anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis. (b) Immunohistochemical staining of CD55 on inflammatory cells in kidney of anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis. (c) Immunohistochemical staining of CD59 on inflammatory cells in kidney of anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis. [Colour figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Detection of complement regulatory proteins expression in kidney by immunohistochemistry Immunohistochemical staining was performed for CD46, CD55 and CD59 on 4‐μm deparaffinized sections of formaldehyde‐fixed renal tissue using rabbit anti‐human CD46 polyclonal antibodies (Abcam, Cambridge, UK; ab108307), mouse anti‐human CD55 monoclonal antibodies (Abcam; ab1422) and rabbit anti‐human CD59 polyclonal antibodies (Sigma‐Aldrich, St Louis, MO, USA; HPA026494) as primary antibodies.

Techniques: Immunohistochemical staining, Staining

Journal: Clinical and Experimental Immunology

Article Title: Complement regulatory proteins in kidneys of patients with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis

doi: 10.1111/cei.13051

Figure Lengend Snippet: Association between the mean optical density of CD46, CD55 and CD59 in the glomeruli and clinicopathological parameters of patients with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis. (a) Association between the mean optical density of CD46 in the glomeruli and the proportion of normal glomeruli. (b) Association between the mean optical density of CD55 in the glomeruli and the proportion of total crescents in renal specimens. (c) Association between the mean optical density of CD59 in the glomeruli and the proportion of total crescents in renal specimens. (d) Association between the mean optical density of CD46 and that of CD55 in the glomeruli. (e) Association between the mean optical density of CD46 and that of CD59 in the glomeruli.

Article Snippet: Detection of complement regulatory proteins expression in kidney by immunohistochemistry Immunohistochemical staining was performed for CD46, CD55 and CD59 on 4‐μm deparaffinized sections of formaldehyde‐fixed renal tissue using rabbit anti‐human CD46 polyclonal antibodies (Abcam, Cambridge, UK; ab108307), mouse anti‐human CD55 monoclonal antibodies (Abcam; ab1422) and rabbit anti‐human CD59 polyclonal antibodies (Sigma‐Aldrich, St Louis, MO, USA; HPA026494) as primary antibodies.

Techniques:

Journal: Clinical and Experimental Immunology

Article Title: Complement regulatory proteins in kidneys of patients with anti‐neutrophil cytoplasmic antibody (ANCA)‐associated vasculitis

doi: 10.1111/cei.13051

Figure Lengend Snippet: Double immunofluorescence staining of CD46, CD55, CD59 and markers of three glomerular intrinsic cells in renal specimens of normal controls. (a–c) Co‐localization of CD46 (red) with CD31 (green), α‐smooth muscle actin (α‐SMA) (green) and podocalyxin (green) in glomeruli. (d–f) Co‐localization of CD55 (red) with CD31 (green), α‐SMA (green) and podocalyxin (green) in glomeruli. (g–i) Co‐localization of CD59 (red) with CD31 (green), α‐SMA (green) and podocalyxin (green) in glomeruli. The specific co‐staining for CD31, α‐SMA, podocalyxin and the three complement regulatory proteins (CRPs) was apparent in the merged picture as yellow/orange in glomeruli. [Colour figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Detection of complement regulatory proteins expression in kidney by immunohistochemistry Immunohistochemical staining was performed for CD46, CD55 and CD59 on 4‐μm deparaffinized sections of formaldehyde‐fixed renal tissue using rabbit anti‐human CD46 polyclonal antibodies (Abcam, Cambridge, UK; ab108307), mouse anti‐human CD55 monoclonal antibodies (Abcam; ab1422) and rabbit anti‐human CD59 polyclonal antibodies (Sigma‐Aldrich, St Louis, MO, USA; HPA026494) as primary antibodies.

Techniques: Double Immunofluorescence Staining, Staining

Journal: Cell Genomics

Article Title: Modular vector assembly enables rapid assessment of emerging CRISPR technologies

doi: 10.1016/j.xgen.2024.100519

Figure Lengend Snippet:

Article Snippet: B2M: APC anti-human B2M antibody (Biolegend, 316312) CD4: APC anti-human CD4 antibody (Biolegend, 357408) CD26 (DPP4): FITC anti-human CD26 antibody (Biolegend, 302704) CD46: FITC anti-human CD46 antibody (Biolegend, 315304) CD47: FITC anti-human CD47 antibody (Biolegend, 323106) CD55: FITC anti-human CD55 antibody (Biolegend, 311305) CD59: FITC anti-human CD59 antibody (Biolegend, 304706) CD63: PE anti-human CD63 antibody (Biolegend, 353004) CD81: APC anti-human CD81 antibody (Biolegend, 349510) CD97 (ADGRE5): FITC anti-human CD97 antibody (Biolegend, 336306) CD274 (PD-L1): APC anti-human CD274 antibody (Biolegend, 329708) LDLR: PE anti-human LDLR antibody (BD Biosciences, 565653) Cells were washed with PBS two times to remove residual antibody and were resuspended in flow buffer (PBS, 2% FBS, 5 μM EDTA).

Techniques: Recombinant, Plasmid Preparation, Software